Other

Part:BBa_K3726108:Design

Designed by: Lucia Tapia   Group: iGEM21_MADRID_UCM   (2021-10-08)


5CON1(H)_NS3-up (PCC 11801)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 143
    Illegal PstI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 143
    Illegal PstI site found at 619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 185
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 143
    Illegal PstI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 143
    Illegal PstI site found at 619
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 850


Design Notes

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a 5’CON part the upstream overhang is AACA , while the downstream overhang is GGAG.


Source

Homology connector has been found within the genome of Synechococcus elongatus PCC 11801.

Genome available at https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2219813